By Rodolfo Paoletti, Dr. David Kritchevsky
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Extra info for Advances in lipid research. Volume 6
For quantitative work band intensities might be corrected for changes in the absorbance of the dye. 90, regeneration may b e accomplished by heating the dye solution to boiling under a condenser, but this regeneration can be done only once. 891 at 37°C. Plasma Lipoprotein 6. 41 Analysis Care ofElectrophoresis Cell An important part of obtaining reproducible lipoprotein electrophoresis is the routine maintenance of the electrophoresis cell. If runs are to occur daily the cell may be kept at room temperature —progressive growth of bacteria or molds has not b e e n noted.
In order to improve the mechanical properties of the gel for subsequent handling, additional monomer and catalyst were diffused into the soft gel after electrophoresis had b e e n completed. , 1966a,b,c; Narayan, 1967a,b; Dudacek and Narayan, 1966). T h e major H D L and L D L components of the sera of man and various laboratory animals can be demonstrated; minor components of uncertain significance have also b e e n seen. / . Agar and Agarose Gels. T h e successful use of gels made from agar and agarose for immunoelectrophoresis (Grabar and Williams, 1955; Scheidegger, 1955; Grabar, 1958, 1959; Grabar and Burtin, 1964) prompted application to lipoproteins.
Plasma Lipoprotein 6. 41 Analysis Care ofElectrophoresis Cell An important part of obtaining reproducible lipoprotein electrophoresis is the routine maintenance of the electrophoresis cell. If runs are to occur daily the cell may be kept at room temperature —progressive growth of bacteria or molds has not b e e n noted. For longer intervals it should be stored at 4°C, allowing sufficient time for rewarming before the next run. T h e central partition and the racks should b e cleaned of buffer deposits and dried b e t w e e n runs.
Advances in lipid research. Volume 6 by Rodolfo Paoletti, Dr. David Kritchevsky