By Rodolfo Paoletti, Dr. David Kritchevsky
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Additional resources for Advances in lipid research. Volume 1
The possibility of ester exchange during hydrolysis was eliminated by subjecting a mixture of synthetic glycerides to hydrolysis, before and after oxidation. These observations, and triplicate determinations made on lard, show that the method is accurate to within ± 2 % of the six glyceride classes estimated. Youngs has reported the results obtained in this way for five natural fats—chicken, rat, pig, cocoa butter, and linseed oil. He finds the fatty acid distributions do not conform with an earlier theory of his own (Youngs, 1959), but agree very well with those calculated by the method of Vander Wal (1960).
Evidence dating from the close of the last century (see Section I,A) has clearly established that natural fats are largely mixtures of mixed triglycérides. B. EVEN (HILDITCH) The theory of even, or widest, distribution was first propounded by Hilditch and his collaborators (Collin and Hilditch, 1929) to describe a pronounced tendency in the distribution of the fatty acids between the glycerides of natural fats. Essentially this theory amounts to the converse of the monoacid theory. Perhaps it is most simply expressed as follows: if an acid forms a third or more of the total acids present, it will occur at least once in every glyceride; if it forms two-thirds it will occur twice; and only when it forms more than two-thirds of the total acids will it appear as a simple triglycéride.
In this way only the methyl esters of the free fatty acids are analyzed. Ast and Vander Wal (1961) have followed a similar proce- Structural Investigation of Natural Fats 27 dure. However, Mattson and Volpenheim (1961a) and Coleman (1963b) have shown that the composition of the fatty acids liberated changes with the extent of the hydrolysis, and for this reason the isolation and analysis of the monoglycerides appears to be a more reliable method than an analysis of the free fatty acids. The point here is that as Coleman and Fulton (1961) have shown glycerol is produced during these hydrolyses, so that some of the free fatty acids must have come from the 2 positions of the original triglycérides.
Advances in lipid research. Volume 1 by Rodolfo Paoletti, Dr. David Kritchevsky